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stable human npffr1 cho  (Revvity)


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    Structured Review

    Revvity stable human npffr1 cho
    NPFF EC50 in functional calcium mobilization assays. (A) NPFF response in stable <t>NPFF1-RD-HGA16</t> cells (●) and parental RD-HGA16 CHO cells (○). (B) NPFF response in stable NPFF2-RD-HGA16 cells (■) and parental RD-HGA16 CHO cells (○). Representative data from one experiment is shown and each data point is the mean ± SD of duplicate determinations.
    Stable Human Npffr1 Cho, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stable+human+npffr1+cho/Neuropeptide+FF+NPFF1+ValiScreen+Cell+Line/pmc06777341-608-0-12
    Average 90 stars, based on 1 article reviews
    stable human npffr1 cho - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Discovery of Novel Proline-Based Neuropeptide FF Receptor Antagonists"

    Article Title: Discovery of Novel Proline-Based Neuropeptide FF Receptor Antagonists

    Journal: ACS chemical neuroscience

    doi: 10.1021/acschemneuro.7b00219

    NPFF EC50 in functional calcium mobilization assays. (A) NPFF response in stable NPFF1-RD-HGA16 cells (●) and parental RD-HGA16 CHO cells (○). (B) NPFF response in stable NPFF2-RD-HGA16 cells (■) and parental RD-HGA16 CHO cells (○). Representative data from one experiment is shown and each data point is the mean ± SD of duplicate determinations.
    Figure Legend Snippet: NPFF EC50 in functional calcium mobilization assays. (A) NPFF response in stable NPFF1-RD-HGA16 cells (●) and parental RD-HGA16 CHO cells (○). (B) NPFF response in stable NPFF2-RD-HGA16 cells (■) and parental RD-HGA16 CHO cells (○). Representative data from one experiment is shown and each data point is the mean ± SD of duplicate determinations.

    Techniques Used: Functional Assay

    Antagonist activity of compound 16 in NPFF1 (A) and NPFF2 (B) calcium mobilization functional Ke assays. (A) Concentration-response curves of NPFF alone (○) and NPFF + 5 μM final 16 (□) in stable NPFF1-RD-HGA16 cells. (B) Concentration-response curves of NPFF alone (○) and NPFF + 10 μM final 16 (□) in stable NPFF2-RD-HGA16 cells. The right shift of the NPFF curve in the presence of test compound was used to calculate Ke values as described in the Methods. Representative data from one experiment are shown and each data point is mean ± SD of duplicate determinations.
    Figure Legend Snippet: Antagonist activity of compound 16 in NPFF1 (A) and NPFF2 (B) calcium mobilization functional Ke assays. (A) Concentration-response curves of NPFF alone (○) and NPFF + 5 μM final 16 (□) in stable NPFF1-RD-HGA16 cells. (B) Concentration-response curves of NPFF alone (○) and NPFF + 10 μM final 16 (□) in stable NPFF2-RD-HGA16 cells. The right shift of the NPFF curve in the presence of test compound was used to calculate Ke values as described in the Methods. Representative data from one experiment are shown and each data point is mean ± SD of duplicate determinations.

    Techniques Used: Activity Assay, Functional Assay, Concentration Assay

    Antagonist activity of compounds 16 and 33 in NPFF1 (A) and FF2 (B) cAMP functional Ke assays. (A) Concentration-response curves of NPFF alone (○), NPFF + 4 μM final 16 (□), and NPFF + 2 μM final 33 (◊) in stable NPFF1-CHO cells. (B) Concentration-response curves of NPFF alone (○), NPFF + 10 μM final 16 (□), and NPFF + 10 μM final 33 (◊) in stable NPFF2-CHO cells. The right shift of the NPFF curve in the presence of test compound was used to calculcate Ke values as described in the Methods. Each data point is mean ± SEM of at least N=3 conducted in duplicate.
    Figure Legend Snippet: Antagonist activity of compounds 16 and 33 in NPFF1 (A) and FF2 (B) cAMP functional Ke assays. (A) Concentration-response curves of NPFF alone (○), NPFF + 4 μM final 16 (□), and NPFF + 2 μM final 33 (◊) in stable NPFF1-CHO cells. (B) Concentration-response curves of NPFF alone (○), NPFF + 10 μM final 16 (□), and NPFF + 10 μM final 33 (◊) in stable NPFF2-CHO cells. The right shift of the NPFF curve in the presence of test compound was used to calculcate Ke values as described in the Methods. Each data point is mean ± SEM of at least N=3 conducted in duplicate.

    Techniques Used: Activity Assay, Functional Assay, Concentration Assay



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    stable human npffr1 cho  (Revvity)
    90
    Revvity stable human npffr1 cho
    NPFF EC50 in functional calcium mobilization assays. (A) NPFF response in stable <t>NPFF1-RD-HGA16</t> cells (●) and parental RD-HGA16 CHO cells (○). (B) NPFF response in stable NPFF2-RD-HGA16 cells (■) and parental RD-HGA16 CHO cells (○). Representative data from one experiment is shown and each data point is the mean ± SD of duplicate determinations.
    Stable Human Npffr1 Cho, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stable+human+npffr1+cho/Neuropeptide+FF+NPFF1+ValiScreen+Cell+Line/pmc06777341-608-0-12
    Average 90 stars, based on 1 article reviews
    stable human npffr1 cho - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    NPFF EC50 in functional calcium mobilization assays. (A) NPFF response in stable NPFF1-RD-HGA16 cells (●) and parental RD-HGA16 CHO cells (○). (B) NPFF response in stable NPFF2-RD-HGA16 cells (■) and parental RD-HGA16 CHO cells (○). Representative data from one experiment is shown and each data point is the mean ± SD of duplicate determinations.

    Journal: ACS chemical neuroscience

    Article Title: Discovery of Novel Proline-Based Neuropeptide FF Receptor Antagonists

    doi: 10.1021/acschemneuro.7b00219

    Figure Lengend Snippet: NPFF EC50 in functional calcium mobilization assays. (A) NPFF response in stable NPFF1-RD-HGA16 cells (●) and parental RD-HGA16 CHO cells (○). (B) NPFF response in stable NPFF2-RD-HGA16 cells (■) and parental RD-HGA16 CHO cells (○). Representative data from one experiment is shown and each data point is the mean ± SD of duplicate determinations.

    Article Snippet: Stable human NPFFR1-CHO (ES-491-C) and NPFFR2-CHO (ES-490-C) cell lines were purchased from PerkinElmer and used with the Lance Ultra kit (TRF0262) to detect cAMP accumulation in low volume 96-well plates.

    Techniques: Functional Assay

    Antagonist activity of compound 16 in NPFF1 (A) and NPFF2 (B) calcium mobilization functional Ke assays. (A) Concentration-response curves of NPFF alone (○) and NPFF + 5 μM final 16 (□) in stable NPFF1-RD-HGA16 cells. (B) Concentration-response curves of NPFF alone (○) and NPFF + 10 μM final 16 (□) in stable NPFF2-RD-HGA16 cells. The right shift of the NPFF curve in the presence of test compound was used to calculate Ke values as described in the Methods. Representative data from one experiment are shown and each data point is mean ± SD of duplicate determinations.

    Journal: ACS chemical neuroscience

    Article Title: Discovery of Novel Proline-Based Neuropeptide FF Receptor Antagonists

    doi: 10.1021/acschemneuro.7b00219

    Figure Lengend Snippet: Antagonist activity of compound 16 in NPFF1 (A) and NPFF2 (B) calcium mobilization functional Ke assays. (A) Concentration-response curves of NPFF alone (○) and NPFF + 5 μM final 16 (□) in stable NPFF1-RD-HGA16 cells. (B) Concentration-response curves of NPFF alone (○) and NPFF + 10 μM final 16 (□) in stable NPFF2-RD-HGA16 cells. The right shift of the NPFF curve in the presence of test compound was used to calculate Ke values as described in the Methods. Representative data from one experiment are shown and each data point is mean ± SD of duplicate determinations.

    Article Snippet: Stable human NPFFR1-CHO (ES-491-C) and NPFFR2-CHO (ES-490-C) cell lines were purchased from PerkinElmer and used with the Lance Ultra kit (TRF0262) to detect cAMP accumulation in low volume 96-well plates.

    Techniques: Activity Assay, Functional Assay, Concentration Assay

    Antagonist activity of compounds 16 and 33 in NPFF1 (A) and FF2 (B) cAMP functional Ke assays. (A) Concentration-response curves of NPFF alone (○), NPFF + 4 μM final 16 (□), and NPFF + 2 μM final 33 (◊) in stable NPFF1-CHO cells. (B) Concentration-response curves of NPFF alone (○), NPFF + 10 μM final 16 (□), and NPFF + 10 μM final 33 (◊) in stable NPFF2-CHO cells. The right shift of the NPFF curve in the presence of test compound was used to calculcate Ke values as described in the Methods. Each data point is mean ± SEM of at least N=3 conducted in duplicate.

    Journal: ACS chemical neuroscience

    Article Title: Discovery of Novel Proline-Based Neuropeptide FF Receptor Antagonists

    doi: 10.1021/acschemneuro.7b00219

    Figure Lengend Snippet: Antagonist activity of compounds 16 and 33 in NPFF1 (A) and FF2 (B) cAMP functional Ke assays. (A) Concentration-response curves of NPFF alone (○), NPFF + 4 μM final 16 (□), and NPFF + 2 μM final 33 (◊) in stable NPFF1-CHO cells. (B) Concentration-response curves of NPFF alone (○), NPFF + 10 μM final 16 (□), and NPFF + 10 μM final 33 (◊) in stable NPFF2-CHO cells. The right shift of the NPFF curve in the presence of test compound was used to calculcate Ke values as described in the Methods. Each data point is mean ± SEM of at least N=3 conducted in duplicate.

    Article Snippet: Stable human NPFFR1-CHO (ES-491-C) and NPFFR2-CHO (ES-490-C) cell lines were purchased from PerkinElmer and used with the Lance Ultra kit (TRF0262) to detect cAMP accumulation in low volume 96-well plates.

    Techniques: Activity Assay, Functional Assay, Concentration Assay